| 產品編號 | bs-48020P |
| 英文名稱 | Recombinant human VEGF-165 protein (Active, HEK293) |
| 中文名稱 | 重組人VEGF-165 (活性的, Tag free) |
| 別 名 | rhVEGF-165; VEGF165; VEGF 165; Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeabilit 人VEGF165; 人VEGF165重組蛋白; 重組人VEGF165; 人重組人VEGF165; 重組人血管內皮生長因子165; |
| 理論分子量 | kDa |
| 檢測分子量 | 39-45 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 27-191/232 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統 | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Vascular endothelial growth factor (VEGF) is a potent growth and angiogenic cytokine. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. Humans express alternately spliced isoforms, and VEGF-165 is the most abundant and potent isoform. VEGF stimulates angiogenesis, vasculogenesis and endothelial cell growth, induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Recombinant human VEGF-165 is a 39-45kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide. This recombinant human VEGF-165 is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
