| 產品編號 | bs-18579R |
| 英文名稱 | LYPLA3 Rabbit pAb |
| 中文名稱 | 溶酶體磷脂酶3抗體 |
| 別 名 | 1 O acylceramide synthase; 1-O-acylceramide synthase; ACS; DKFZp564A0122; Group XV phospholipase A2; GXVPLA 2; GXVPLA2; LCAT like lysophospholipase; LCAT-like lysophospholipase; LLPL; LPLA 2; LPLA2; LYPLA 3; Lysophospholipase 3(lysosomal phospholipase A2); Lysophospholipase 3; Lysosomal phospholipase A2; OTTHUMP00000174859; PAG15_HUMAN; phospholipase A2, group XV; PLA2G15; UNQ341/PRO540. |
| 研究領域 | 腫瘤 細胞生物 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LYPLA3: 101-200/412 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq, Jul 2008] Function: Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity. Subcellular Location: Lysosome. Secreted. Tissue Specificity: Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Post-translational modifications: N-glycosylated. Similarity: Belongs to the AB hydrolase superfamily. Lipase family. SWISS: Q8NCC3 Gene ID: 23659 Database links: Entrez Gene: 23659 Human Entrez Gene: 192654 Mouse Omim: 609362 Human SwissProt: Q8NCC3 Human SwissProt: Q8VEB4 Mouse Unigene: 632199 Human Unigene: 284770 Mouse Unigene: 93631 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human HeLa cell lysates
Primary: Anti-LYPLA3 (bs-18579R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZC3HAV1L) Polyclonal Antibody, Unconjugated (bs-18531R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
