| 產品編號 | bs-14508R |
| 英文名稱 | phospho-EEF1A2（Ser358） Rabbit pAb |
| 中文名稱 | 磷酸化翻譯延伸因子EF-1 α2抗體 |
| 別 名 | EEF1A2(phospho S358); p-EEF1A2(phospho S358); eEF1A 2; eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 信號轉導 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human EEF1A2 around the phosphorylation site of Ser358: GY(p-S)PV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subcellular Location: Nucleus. Tissue Specificity: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. SWISS: Q05639 Gene ID: 1917 Database links: Entrez Gene: 1917 Human Entrez Gene: 13628 Mouse Entrez Gene: 101109898 Sheep Entrez Gene: 380550 Xenopus laevis Omim: 602959 Human SwissProt: Q05639 Human SwissProt: P62631 Mouse Unigene: 433839 Human Unigene: 2645 Mouse Unigene: 9764 Rat |
| 產品圖片 |
Sample: HL-60 Cell (Human) Lysate at 40 ug
Primary: Anti- phospho-EEF1A2（Ser358） (bs-14508R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
