| 產品編號 | bs-11842R |
| 英文名稱 | Frizzled 9 Rabbit pAb |
| 中文名稱 | CD349抗體 |
| 別 名 | CD 349; CD 349 antigen; CD349; Frizzled Drosophila homolog of 3 Frizzled homolog 9(Drosophila); Frizzled homolog 9; Frizzled-9; Fz 9; Fz-9; FZD 3; Fzd 9; FZD3; FZD3-PEN; Fzd9; FZD9_HUMAN; FzE6; hFz9; ymfz9; Xfz9. |
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Specific References (1) | bs-11842R has been referenced in 1 publications.
[IF=1] Vasyliev, R. G., et al. "Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications." Experimental Oncology 39.3 (2017): 171-180. FCM ; Human.
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| 研究領域 | 細胞生物 神經生物學 信號轉導 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Dog) |
| 產品應用 | WB=1:500-2000,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FZD9 protein: 469-591/591 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008] Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Subcellular Location: Cell membrane. Tissue Specificity: Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney. Post-translational modifications: Ubiquitinated by ZNRF3, leading to its degradation by the proteasome Similarity: Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain. SWISS: O00144 Gene ID: 8326 Database links: Entrez Gene: 8326 Human Entrez Gene: 14371 Mouse Omim: 601766 Human SwissProt: O00144 Human SwissProt: Q9R216 Mouse Unigene: 647029 Human Unigene: 6256 Mouse |
| 產品圖片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti- Frizzled 9 (bs-11842R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 64 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
