| 產品編號 | bs-8326R |
| 英文名稱 | Hi95 Rabbit pAb |
| 中文名稱 | 缺氧誘導基因95抗體 |
| 別 名 | Hi95; Hypoxia induced gene 95; MGC11758; OTTHUMP00000003641; RP23 63D8.3; SES2; SESN 2; SEST2; Sestrin 2; DKFZp761M02121; OTTMUSP00000010184; DKFZp761M0212; SESN2_HUMAN. |
| 研究領域 | 心血管 細胞生物 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SESN2/Hi95: 101-200/480 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SESN2 is a member of the sestrin family of PA26-related proteins. The It may function in the regulation of cell growth and survival and also in cellular response to different stress conditions. Function: Involved in the reduction of peroxiredoxins. Subcellular Location: Cytoplasmic and Nuclear Tissue Specificity: Widely expressed. Similarity: Belongs to the sestrin family. SWISS: P58004 Gene ID: 83667 Database links: Entrez Gene: 83667 Human Entrez Gene: 230784 Mouse Omim: 607767 Human SwissProt: P58004 Human SwissProt: P58043 Mouse Unigene: 469543 Human Unigene: 23608 Mouse Unigene: 37672 Rat |
| 產品圖片 |
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (bs-8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (bs-8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
