| 產品編號 | bs-7861R |
| 英文名稱 | EME1 Rabbit pAb |
| 中文名稱 | 減數分裂內切酶EME1抗體 |
| 別 名 | Crossover junction endonuclease EME1; Eme1 essential meiotic endonuclease 1 homolog 1(S. pombe); EME1, S. pombe, homolog of, 1; essential meiotic endonuclease 1 homolog 1(S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1, S. pombe, homolog of, 1; hMMS4; homolog of yeast EME1 endonuclease; MMS4; MMS4 homolog; MMS4L; EME1_HUMAN. |
| 研究領域 | 細胞生物 發育生物學 干細胞 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EME1: 412-500/570 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
EME1 complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein (MUS81) to form an endonuclease complex which cleaves branched DNA structures, especially those arising during stalled DNA replication. The protein may be involved in repairing DNA damage and in maintaining genomic stability. It interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Alternative splicing results in multiple transcript variants. Function: Interacts with MUS81 to form a DNA structure-specificendonuclease with substrate preference for branched DNA structureswith a 5'-end at the branch nick. Typical substrates include3'-flap structures, replication forks and nicked Hollidayjunctions. May be required in mitosis for the processing of stalledor collapsed replication forks. Subunit: May self-associate. Interacts with MUS81. Interacts withERCC4 and FANCM. Subcellular Location: Nucleus, nucleolus. Note=Recruited toregions of DNA damage in S-phase cells. Similarity: Belongs to the EME1/MMS4 family. SWISS: Q96AY2 Gene ID: 146956 Database links: UniProtKB/Swiss-Prot: Q96AY2.2 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
