| 產品編號 | bs-6376R |
| 英文名稱 | DDIT4L Rabbit pAb |
| 中文名稱 | DNA損傷誘導轉錄樣蛋白4抗體 |
| 別 名 | DNA damage inducible transcript 4 like protein; HIF 1 responsive protein RTP801 like; Homolog of mouse SMHS1; Protein regulated in development and DNA damage response 2; REDD 2; REDD2; RTP801L; DDT4L_HUMAN. |
| 研究領域 | 細胞生物 免疫學 細胞周期蛋白 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDIT4L: 121-193/193 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DDIT4L inhibits cell growth by regulating the FRAP1 pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. It is up regulated in atherosclerotic plaques relative to healthy segments of the same artery. Function: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Subcellular Location: Cytoplasm. Tissue Specificity: Up-regulated in atherosclerotic plaques relative to healthy segments of the same artery. Similarity: Belongs to the DDIT4 family. SWISS: Q96D03 Gene ID: 115265 Database links: Entrez Gene: 115265 Human Entrez Gene: 73284 Mouse Omim: 607730 Human SwissProt: Q96D03 Human SwissProt: Q8VHZ5 Mouse Unigene: 480378 Human Unigene: 250841 Mouse Unigene: 19672 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDIT4L) Polyclonal Antibody, Unconjugated (bs-6376R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
