| 產品編號 | bs-5720R |
| 英文名稱 | GDF10 Rabbit pAb |
| 中文名稱 | 生長分化因子10抗體 |
| 別 名 | BIP; BMP 3b; BMP3B; Bone inducing protein; Bone morphogenetic protein 3b; GDF 10; GDF-10; growth differentiation factor 10; BMP3B_HUMAN; Bone-inducing protein; Growth/differentiation factor 10. |
 |
Specific References (1) | bs-5720R has been referenced in 1 publications.
[IF=15.266] Armaka, Marietta. et al. Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis. GENOME MED. 2022 Dec;14(1):1-25 IF ; Mouse.
|
| 研究領域 | 腫瘤 免疫學 信號轉導 干細胞 細胞周期蛋白 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12/53 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDF10: 371-478/478 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GDF10, or growth differentiation factor 10, bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. GDF10 is expressed in femur, brain, lung, skeletal muscle, pancreas and testis. Subcellular Location: Secreted. Tissue Specificity: Expressed in femur, brain, lung, skeletal muscle, pancreas and testis. Similarity: Belongs to the TGF-beta family. SWISS: P55107 Gene ID: 2662 Database links: Entrez Gene: 2662 Human Entrez Gene: 14560 Mouse GenBank: NM_004962 Human Omim: 601361 Human SwissProt: P55107 Human SwissProt: P97737 Mouse Unigene: 2171 Human Unigene: 432071 Mouse |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Pancreas tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-GDF10 (bs-5720R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 53 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDF10) Polyclonal Antibody, Unconjugated (bs-5720R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
