| 產品編號 | bs-5685R |
| 英文名稱 | phospho-RUNX2 (Ser451) Rabbit pAb |
| 中文名稱 | 磷酸化成骨特異性轉錄因子抗體 |
| 別 名 | Runx-2; RUNX2(phospho S451); Runt-related Transcription Factor 2; CBF alpha 1; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit. |
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Specific References (6) | bs-5685R has been referenced in 6 publications.
[IF=9.078] Tzu-Hsiang Lin. et al. A bilineage thermosensitive hydrogel system for stimulation of mesenchymal stem cell differentiation and enhancement of osteochondral regeneration. Compos Part B-Eng. 2022 Jan;:109614 ICC ; Rabbit.
[IF=5.168] Kim et al. Characterization of CADD522, a small molecule that inhibits RUNX2-DNA binding and exhibits antitumor activity. (2017) Oncotarget. 8:70916-70940 WB ; Human.
[IF=4.165] Yu-Ting Yen. et al. PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation. Commun Biol. 2021 Jun;4(1):1-12 WB,IHC ; Mouse.
[IF=3.201] Nagy A et al. Zinc Inhibits HIF-Prolyl Hydroxylase Inhibitor-Aggravated VSMC Calcification Induced by High Phosphate. Front Physiol. 2020 Jan 15;10:1584. WB ; Human.
[IF=3.08] Wang et al. Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling. (2016) Mol.Cells. 39:186-94 FC ; Human.
[IF=0] Schlie-Wolter, S., et al. Osteogenic Differentiation of Human Adipose-Derived Stem Cells (hASC): Role of FAK and Ras-MAPK Signaling. (2016) J Stem Cell Regen Bio 2(2): 1- 19 other ; Human.
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| 產品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 干細胞 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human RUNX2 around the phosphorylation site of Ser451: TS(p-S)GS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. Subunit: Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4. Subcellular Location: Nucleus. Tissue Specificity: Specifically expressed in osteoblasts. Similarity: Contains 1 Runt domain. SWISS: Q13950 Gene ID: 860 Database links: Entrez Gene: 860 Human Entrez Gene: 12393 Mouse Omim: 600211 Human SwissProt: Q13950 Human SwissProt: Q9XSB7 Horse SwissProt: Q08775 Mouse Unigene: 535845 Human Unigene: 391013 Mouse Unigene: 391017 Mouse Unigene: 214214 Rat Unigene: 83672 Rat RUNX2又稱：Cbfα1(Core-binding factor, alpha 3 subunit) 是新發現的一類調控間充質干細胞向成骨方向分化的特異性轉錄因子,參與骨形成,骨骼生長和發育的一類重要細胞,它起源于多能間充質干細胞,是間充質干細胞在體內的各種調控因素的調節下發育而成的。 |
| 產品圖片 |
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-phospho-RUNX2 (Ser451) antibody (bs-5685R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
