| 產品編號 | bs-5541R |
| 英文名稱 | phospho-PTK2 (Tyr576+Tyr577) Rabbit pAb |
| 中文名稱 | 磷酸化粘著斑激酶抗體 |
| 別 名 | PTK2(phospho Y576/Y577); FAK(phospho Y576+Y577); FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 免疫學 信號轉導 細胞凋亡 轉錄調節因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 119 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr576/577: ST(p-Y)(p-Y)KA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions. Subcellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Tissue Specificity: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. SWISS: Q05397 Gene ID: 5747 Database links: Entrez Gene: 5747 Human Entrez Gene: 14083 Mouse Omim: 600758 Human SwissProt: Q05397 Human SwissProt: P34152 Mouse Unigene: 395482 Human Unigene: 254494 Mouse Unigene: 2809 Rat FAK是整合蛋白介導的信號轉導中的重要成員,有酪氨酸蛋白激酶活性,并可自身磷酸化,FAK本身是胱冬肽酶(caspase)的底物。作為信號分子的FAK參與抑制細胞凋亡并直接參與細胞多種功能的調節。 1.FAK 局部粘著斑激酶，是一種酪氨酸激酶;腫瘤細胞的侵襲性生長是一個多步驟的復雜過程，有多種生物化學因子參與其中,局部粘著斑激酶（focal adhesion kinase, FAK）介導的信號轉導系統就是其中最為重要的細胞信號轉導途徑之一。腫瘤細胞必須黏附于細胞外基質，通過促進依賴于PTK激酶活性的細胞外基質信號轉導，進而影響細胞的黏附、運動與遷移。 2.粘著斑激酶(focal adhesion kinase,FAK)是整合蛋白介導的信號轉導中的重要成員,有酪氨酸蛋白激酶活性,并可自身磷酸化；為信號分子的FAK,還與細胞內其他信號轉導通路存在串話(crosstalk),直接參與了細胞多種功能的調節。 3.盡管FAK的確切功能尚不清楚,但若干實驗均提示FAK可能有兩個作用,一是在細胞鋪展和移動時,FAK參與粘著斑形成和調節；二是FAK參與信號轉導過程,以告知細胞核其細胞已錨定了。近年有關FAK在細胞凋亡中的作用也業已肯定。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PTK2 (Tyr576+Tyr577)) Polyclonal Antibody, Unconjugated (bs-5541R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
