| 產品編號 | bs-5495R |
| 英文名稱 | phospho-MAP3K7IP1 (Ser423) Rabbit pAb |
| 中文名稱 | 磷酸化轉化生長因子β活化激酶結合蛋白1抗體 |
| 別 名 | MAP3K7IP1(phospho S423); MAP3K7IP1(phospho S421) mo, rat; MAP3K7IP 1; MAP3K7IP1; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; TAB 1; TAK1 binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; Transforming growth factor beta activated kinase binding protein 1; 2310012M03Rik; 3'-Tab1; MGC57664; TAB1_HUMAN. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 轉錄調節因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MAP3K7IP1 around the phosphorylation site of Ser423: AH(p-S)AS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta and members of the Toll IL 1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N terminus acts as a dominant negative inhibitor of TGF beta, demonstrating how this protein can function as a mediator between TGF beta receptors and TAK1. Function: May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis. Subunit: Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2. Tissue Specificity: Ubiquitous. Post-translational modifications: Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP. Similarity: Contains 1 PP2C-like domain. SWISS: Q15750 Gene ID: 10454 Database links: Entrez Gene: 10454 Human Entrez Gene: 66513 Mouse Omim: 602615 Human SwissProt: Q15750 Human SwissProt: Q8CF89 Mouse Unigene: 507681 Human Unigene: 288245 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAP3K7IP1 (Ser423)) Polyclonal Antibody, Unconjugated (bs-5495R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
