| 產品編號 | bs-5303R |
| 英文名稱 | phospho-DAAM1 (Thr361) Rabbit pAb |
| 中文名稱 | 磷酸化形態發生紊亂關聯激活因子1抗體 |
| 別 名 | DAAM 1; DAAM-1; Dishevelled associated activator of morphogenesis 1; FLJ41657; KIAA0666. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Chicken,Dog,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 120-130 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human DAAM1 around the phosphorylation site of Thr361: SA(p-T)QM |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling. Function: Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Subunit: Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Subcellular Location: Cytoplasm. Note=Perinuclear. Tissue Specificity: Expressed in all tissues examined. Similarity: Belongs to the formin homology family. Contains 1 DAD (diaphanous autoregulatory) domain. Contains 1 FH1 (formin homology 1) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. SWISS: Q9Y4D1 Gene ID: 23002 Database links: Entrez Gene: 23002 Human Entrez Gene: 208846 Mouse Omim: 606626 Human SwissProt: Q9Y4D1 Human SwissProt: Q8BPM0 Mouse Unigene: 654934 Human DAAM1是蓬亂蛋白相關形態形成活化因子1或稱形態發生紊亂關聯激活因子1，為形成素蛋白家族的成員。Daam1是WNT/PCP通路的組成因子之一,參與細胞的內吞功能,可調節細胞骨架的形成與構建. |
| 產品圖片 |
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-DAAM1 (Thr361) (bs-5303R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120-130 kD
Observed band size: 125 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
